We use Sass to compile our CSS. Typically we use the em and % units for everything.
Gulp can be used to add browser prefixes and to compile your Sass files.
Respond is a library that allows IE6-8 to read media queries, which is essential since we are using a grid system. This should only be loaded conditionally if you are trying to support those browsers.
Modernizr is used for feature detection. It also ships with html5shiv.js which enables HTML5 elements in IE.
We use the basic grid logic from the Bourbon's Neat library in order to create our grid system.