- Grab the code
$ git clone [email protected]:MGXlab/virus_identification_tools_benchmarking.git
$ conda create -n virbench --file conda-linux-64.lock
- Define a tab-separated samplesheet with a header and column names
sample_id
pair_id
: microbial and viral paired idfraction
:viral
ormicrobial
(can be anything really)R1
: Path to forward fastq fileR2
: Path to reverse fastq fileR1_MD5
: MD5 numbers of Generated R1 FASTQ filesR2_MD5
: MD5 numbers of Generated R2 FASTQ files
-
Fill in the
config.yaml
based on your needs. -
Execute from within this dir.
# This is a dry run
(virbench)$ snakemake -j 32 --use-conda --conda-frontend mamba -p -n
- Remove
-n
to run it
Short, paired-end sequencing reads for the viral and microbial fraction of a
sample. The fraction
information must be provided in the samplesheet.